首页> 外文OA文献 >An intron in the thymidylate synthase gene of Bacillus bacteriophage beta 22: evidence for independent evolution of a gene, its group I intron, and the intron open reading frame.

【2h】

An intron in the thymidylate synthase gene of Bacillus bacteriophage beta 22: evidence for independent evolution of a gene, its group I intron, and the intron open reading frame.

机译：杆菌噬菌体β22的胸苷酸合酶基因中的内含子：一个基因，其第I组内含子和内含子开放阅读框的独立进化的证据。

代理获取

本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。

页面导航

摘要
著录项
相似文献
相关主题

摘要

The thymidylate synthase gene (thy) (EC 2.1.1.45) of Bacillus subtilis bacteriophage beta 22 has a self-splicing, group I intron inserted into a highly conserved region of the coding sequence. The intron is very similar to one that is inserted 21 bp further downstream in the homologous thymidylate synthase gene (td) of Escherichia coli bacteriophage T4. In contrast, the amino acid sequences of the bacteriophage thymidylate synthases are highly divergent. The beta 22 intron has a fragmentary open reading frame (ORF) that encodes a putative helix-turn-helix DNA-binding motif, similar to one at the carboxyl terminus of the homing endonuclease (I-TevI) encoded by the T4 td intron. The td ORF and the thy ORF fragments are inserted into different regions of their respective intron structures. These results suggest that the thymidylate synthase genes, their introns, and their respective intron-ORFs all have separate evolutionary histories and that the acquisition of the intron could not have occurred by a simple homing event.

机译：枯草芽孢杆菌噬菌体β22的胸苷酸合酶基因（thy）（EC 2.1.1.45）具有一个自我剪接的I组内含子，插入了编码序列的高度保守区域。该内含子与在大肠杆菌噬菌体T4的同源胸苷酸合酶基因（td）下游21bp处插入的内含子非常相似。相反，噬菌体胸苷酸合酶的氨基酸序列高度不同。 beta 22内含子具有一个片段开放阅读框（ORF），其编码一个推定的螺旋-转-螺旋DNA结合基序，类似于由T4 td内含子编码的归巢核酸内切酶（I-TevI）羧基末端的一个。 td ORF和thy ORF片段被插入各自内含子结构的不同区域。这些结果表明，胸苷酸合酶基因，它们的内含子和它们各自的内含子ORF都具有独立的进化历史，并且通过简单的归巢事件不可能获得内含子。

著录项

作者
Bechhofer, D H; Hue, K K; Shub, D A;
展开▼
作者单位

展开▼
年度 1994
总页数
原文格式 PDF
正文语种 en
中图分类

相似文献

外文文献
中文文献
专利

1. Interrupted thymidylate synthase gene of bacteriophages T2 and T6 and other potential self-splicing introns in the T-even bacteriophages. [J] . F K Chu, F Maley, J Martinez, Journal of bacteriology . 1987,第9期

机译：中断噬菌体T2和T6的胸苷合成酶基因以及在T型均匀噬菌体中的其他潜在的自分割内含子。
2. Interrupted thymidylate synthase gene of bacteriophages T2 and T6 and other potential self-splicing introns in the T-even bacteriophages. [J] . F K Chu, F Maley, J Martinez, Journal of bacteriology . 1987,第9期

机译：中断噬菌体T2和T6的胸苷合成酶基因以及在T型均匀噬菌体中的其他潜在的自分割内含子。
3. Interrupted thymidylate synthase gene of bacteriophages T2 and T6 and other potential self-splicing introns in the T-even bacteriophages. [J] . F K Chu, F Maley, J Martinez, Journal of bacteriology . 1987,第9期

机译：中断噬菌体T2和T6的胸苷合成酶基因以及在T型均匀噬菌体中的其他潜在的自分割内含子。
4. A High Percentage of Introns in Human Genes Were Present Early in Animal Evolution: Evidence from the Basal Metazoan Nematostella vectensis [C] . James C. Sullivan, Adam M. Reitzel, John R. Finnert International Workshop on Bioinformatics and Systems Biology . 2006

机译：人类基因中的高百分比内含子在动物演化中呈现：来自基础甲卓劳埃斯氏菌的证据vectensis
5. A novel intron-encoded endonuclease derived from the fourth intron of the Chlamydomonas reinhardtii psbA gene. [D] . Kim, Hyong-Ha. 2000

机译：从莱茵衣藻psbA基因的第四个内含子衍生的新型内含子编码的核酸内切酶。
6. An intron in the thymidylate synthase gene of Bacillus bacteriophage beta 22: evidence for independent evolution of a gene its group I intron and the intron open reading frame. [O] . D H Bechhofer, K K Hue, D A Shub 1994

机译：芽孢杆菌噬菌体β22的胸苷酸合酶基因中的内含子：一个基因其第一类内含子和内含子开放阅读框的独立进化的证据。
7. Interrupted thymidylate synthase gene of bacteriophages T2 and T6 and other potential self-splicing introns in the T-even bacteriophages. [O] . Chu, F K, Maley, F, Martinez, J, 1987

机译：T-even噬菌体中T2和T6噬菌体的胸苷酸合酶基因和其他潜在的自我剪接内含子被打断。

An intron in the thymidylate synthase gene of Bacillus bacteriophage beta 22: evidence for independent evolution of a gene, its group I intron, and the intron open reading frame.

摘要

著录项

相似文献

相关主题

期刊订阅